@article{Da_Silva_Filho_J-2024_185182,
title = {A spatially resolved single-cell lung atlas integrated with clinical and blood signatures distinguishes COVID-19 disease trajectories},
author = {Da Silva Filho, J. and Herder, V. and Gibbins, M. and Dos Reis, M. and Melo, G. and Haley, M. and Judice, C. and Val, F. and Borba, M. and Tavella, T. and de Sousa Sampaio, V. and Attipa, C. and McMonagle, F. and Wright, D. and de Lacerda, M. and Costa, F. and Couper, K. and Marcelo Monteiro, W. and de Lima Ferreira, L. and Moxon, C. and Palmarini, M. and Marti, M.},
month = {sep},
year = {2024},
abstract = {COVID-19 is characterized by a broad range of symptoms and disease trajectories. Understanding the correlation between clinical biomarkers and lung pathology during acute COVID-19 is necessary to understand its diverse pathogenesis and inform more effective treatments. Here, we present an integrated analysis of longitudinal clinical parameters, peripheral blood markers, and lung pathology in 142 Brazilian patients hospitalized with COVID-19. We identified core clinical and peripheral blood signatures differentiating disease progression between patients who recovered from severe disease compared with those who succumbed to the disease. Signatures were heterogeneous among fatal cases yet clustered into two patient groups: "early death" (&lt;15 days until death) and "late death" (&gt;15 days). Progression to early death was characterized systemically and in lung histopathological samples by rapid endothelial and myeloid activation and the presence of thrombi associated with SARS-CoV-2+ macrophages. In contrast, progression to late death was associated with fibrosis, apoptosis, and SARS-CoV-2+ epithelial cells in postmortem lung tissue. In late death cases, cytotoxicity, interferon, and T helper 17 (TH17) signatures were only detectable in the peripheral blood after 2 weeks of hospitalization. Progression to recovery was associated with higher lymphocyte counts, TH2 responses, and anti-inflammatory-mediated responses. By integrating antemortem longitudinal blood signatures and spatial single-cell lung signatures from postmortem lung samples, we defined clinical parameters that could be used to help predict COVID-19 outcomes.},
volume = {16},
issue = {764},
journal = {Science Translational Medicine},
publisher = {American Association for the Advancement of Science},
url = {https://doi.org/10.1126/scitranslmed.adk9149},
}
