TY - JOUR
AU - Mishra, Neha
AU - Tran, Florian
AU - Bahmer, Thomas
AU - Best, Lena
AU - Bordoni, Dora
AU - Franzenburg, Jeanette
AU - Geisen, Ulf
AU - Josephs-Spaulding, Jonathan
AU - Köhler, Philipp
AU - Künstner, Axel
AU - Rosati, Elisa
AU - Bacher, Petra
AU - Baran, Nathan
AU - Boysen, Teide
AU - Brandt, Burkhard
AU - Bruse, Niklas
AU - Dörr, Jonathan
AU - Dräger, Andreas
AU - Elke, Gunnar
AU - Ellinghaus, David
AU - Fischer, Julia
AU - Forster, Michael
AU - Franke, Andre
AU - Franzenburg, Sören
AU - Frey, Norbert
AU - Friedrichs, Anette
AU - Fuß, Janina
AU - Glück, Andreas
AU - Hamm, Jacob
AU - Hinrichsen, Finn
AU - Imm, Simon
AU - Junker, Ralf
AU - Kaiser, Sina
AU - Knoll, Rainer
AU - Lange, Christoph
AU - Laue, Georg
AU - Lier, Clemens
AU - Lindner, Matthias
AU - Marinos, Georgios
AU - Markewitz, Robert
AU - Nattermann, Jacob
AU - Noth, Rainer
AU - Pickkers, Peter
AU - Renz, Alina
AU - Röcken, Christoph
AU - Rupp, Jan
AU - Schaffarzyk, Annika
AU - Scheffold, Alexander
AU - Schulte-Schrepping, Jonas
AU - Schunk, Domagoj
AU - Skowasch, Dirk
AU - Ulas, Thomas
AU - Wandinger, Klaus-Peter
AU - Wittig, Michael
AU - Zimmermann, Johannes
AU - Busch, Hauke
AU - Kaleta, Christoph
AU - Heyckendorf, Jan
AU - Kox, Matthijs
AU - Rybniker, Jan
AU - Schreiber, Stefan
AU - Rosenstiel, Philip
AU - HCA Lung Biological Network
AU - Deutsche COVID-19 Omics Initiative (DeCOI)
AU - Bernardes, Joana P.
AU - Blase, Johanna I.
AU - Aschenbrenner, Anna C.
AU - Hoeppner, Marc P.
AU - Kan, Ying H.
AU - Rabe, Klaus F.
AU - Hoyer, Bimba F.
AU - Schultze, Joachim L.
PY - 2020
DA - November
TI - Longitudinal multi-omics analyses identify responses of megakaryocytes, erythroid cells, and plasmablasts as hallmarks of severe COVID-19
JO - Immunity
EP - 1314
VL - 53
IS - 6
DO - http://dx.doi.org/10.1016/j.immuni.2020.11.017
AB - Temporal resolution of cellular features associated with a severe COVID-19 disease trajectory is needed for understanding skewed immune responses and defining predictors of outcome. Here, we performed a longitudinal multi-omics study using a two-center cohort of 14 patients. We analyzed the bulk transcriptome, bulk DNA methylome, and single-cell transcriptome (&gt;358,000 cells, including BCR profiles) of peripheral blood samples harvested from up to 5 time points. Validation was performed in two independent cohorts of COVID-19 patients. Severe COVID-19 was characterized by an increase of proliferating, metabolically hyperactive plasmablasts. Coinciding with critical illness, we also identified an expansion of interferon-activated circulating megakaryocytes and increased erythropoiesis with features of hypoxic signaling. Megakaryocyte- and erythroid-cell-derived co-expression modules were predictive of fatal disease outcome. The study demonstrates broad cellular effects of SARS-CoV-2 infection beyond adaptive immune cells and provides an entry point toward developing biomarkers and targeted treatments of patients with COVID-19.
PB - Elsevier
UR - https://eprints.gla.ac.uk/363448/
KW - Coronavirus (COVID-19)
ER