TY - JOUR
AU - Mosavie, Mia
AU - Rynne, Jennifer
AU - Fish, Matthew
AU - Smith, Peter
AU - Jennings, Aislinn
AU - Singh, Shivani
AU - Millar, Jonathan E.
AU - Harvala, Heli
AU - Mora, Ana
AU - Kaloyirou, Fotini
AU - Griffiths, Alexandra
AU - Hopkins, Valerie
AU - Washington, Charlotte
AU - Estcourt, Lise J
AU - Roberts, David
AU - Shankar-Hari, Manu
PY - 2024
DA - September
TI - Changes in Phenotypic and Molecular Features of Naïve and Central Memory T Helper Cell Subsets following SARS-CoV-2 Vaccination
JO - Vaccines
VL - 12
IS - 9
DO - https://doi.org/10.3390/vaccines12091040
AB - Molecular changes in lymphocytes following SARS-CoV-2 vaccination are incompletely understood. We hypothesized that studying the molecular (transcriptomic, epigenetic, and T cell receptor (TCR) repertoire) changes in CD4 + T cells following SARS-CoV-2 vaccination could inform protective mechanisms and refinement of future vaccines. We tested this hypothesis by reporting alterations in CD4 + T cell subsets and molecular features of CD4 + naïve and CD4 + central memory (CM) subsets between the unvaccinated and vaccinated groups. Compared with the unvaccinated, the vaccinated had higher HLA-DR expression in CD4 + T subsets, a greater number of differentially expressed genes (DEGs) that overlapped with key differentially accessible regions (DARs) along the chromatin linked to inflammasome activation, translation, regulation (of apoptosis, inflammation), and significant changes in clonal architecture beyond SARS-CoV-2 specificity. Several of these differences were more pronounced in the CD4 +CM subset. Taken together, our observations imply that the COVID-19 vaccine exerts its protective effects via modulation of acute inflammation to SARS-CoV-2 challenge.
PB - Multidisciplinary Digital Publishing Institute (MDPI)
UR - https://www.research.ed.ac.uk/en/publications/1401607f-97b5-4290-8f0a-5f68475650bb
KW - Coronavirus (COVID-19)
KW - Immunisation and screening
ER