@article{Kurkowiak_M-2023_157331,
title = {Differential RNA editing landscapes in host cell versus the SARS-CoV-2 genome},
author = {Kurkowiak, M. and Fletcher, S. and Daniels, A. and Mozolewski, P. and Silvestris, D. and Król, E. and Marek-Trzonkowska, N. and Hupp, T. and Tait-Burkard, C.},
month = {nov},
year = {2023},
abstract = {The SARS-CoV-2 pandemic was defined by the emergence of new variants formed through virus mutation originating from random errors not corrected by viral proofreading and/or the host antiviral response introducing mutations into the viral genome. While sequencing information hints at cellular RNA editing pathways playing a role in viral evolution, here, we use an in vitro human cell infection model to assess RNA mutation types in two SARS-CoV-2 strains representing the original and the alpha variants. The variants showed both different cellular responses and mutation patterns with alpha showing higher mutation frequency with most substitutions observed being C-U, indicating an important role for apolipoprotein B mRNA editing catalytic polypeptide-like editing. Knockdown of select APOBEC3s through RNAi increased virus production in the original virus, but not in alpha. Overall, these data suggest a deaminase-independent anti-viral function of APOBECs in SARS-CoV-2 while the C-U editing itself might function to enhance genetic diversity enabling evolutionary adaptation.},
pages = {1-19 },
volume = {26},
issue = {11 },
journal = {iScience},
publisher = {Cell Press},
url = {https://doi.org/10.1016/j.isci.2023.108031},
}
