@article{Dong_Y-2022_60152,
title = {Multiplex, variant-tolerant, real-time RT-LAMP for SARS-CoV-2 detection using HFman probe},
author = {Dong, Y. and Zhao, Y. and Li, S. and Wan, Z. and Lu, R. and Yang, X. and Yu, G. and Reboud, J. and Cooper, J. and Tian, Z. and Zhang, C.},
month = {feb},
year = {2022},
abstract = {Viral evolution impacts diagnostic test performance through the emergence of variants with sequences affecting the efficiency of primer binding. Such variants that evade detection by nucleic acid-based tests are subject to selective pressure, enabling them to spread more efficiently. Here, we report a variant-tolerant diagnostic test for SARS-CoV-2 using a loop-mediated isothermal nucleic acid-based amplification (LAMP) assay containing high-fidelity DNA polymerase and a high-fidelity DNA polymerase-medicated probe (HFman probe). In addition to demonstrating a high tolerance to variable SARS-CoV-2 viral sequences, the mechanism also overcomes frequently observed limitations of LAMP assays arising from non-specific amplification within multiplexed reactions performed in a single “pot”. Results showed excellent clinical performance (sensitivity 94.5%, specificity 100%, n = 190) when compared directly to a commercial gold standard reverse transcription quantitative polymerase chain reaction assay for the extracted RNA from nasopharyngeal samples and the capability of detecting a wide range of sequences containing at least alpha and delta variants. To further validate the test with no sample processing, directly from nasopharyngeal swabs, we also detected SARS-CoV-2 in positive clinical samples (n = 49), opening up the possibility for the assay’s use in decentralized testing.},
pages = {730-739},
volume = {7},
journal = {ACS Sensors},
publisher = {American Chemical Society},
url = {http://dx.doi.org/10.1021/acssensors.1c02079},
}
