TY - JOUR
AU - Stephenson, Emily
AU - Reynolds, Gary
AU - Botting, Rachel A.
AU - Calero-Nieto, Fernando J.
AU - Morgan, Michael D.
AU - Tuong, Zewen Kelvin
AU - Bach, Karsten
AU - Sungnak, Waradon
AU - Worlock, Kaylee B.
AU - Yoshida, Masahiro
AU - Kumasaka, Natsuhiko
AU - Kania, Katarzyna
AU - Engelbert, Justin
AU - Olabi, Bayanne
AU - Spegarova, Jarmila Stremenova
AU - Mende, Nicole
AU - Jardine, Laura
AU - Gardner, Louis C. S.
AU - Goh, Issac
AU - Horsfall, Dave
AU - McGrath, Jim
AU - Webb, Simone
AU - Mather, Michael W.
AU - Lindeboom, Rik G. H.
AU - Dann, Emma
AU - Huang, Ni
AU - Polanski, Krzysztof
AU - Prigmore, Elena
AU - Gothe, Florian
AU - Scott, Jonathan
AU - Payne, Rebecca P.
AU - Baker, Kenneth F.
AU - Hanrath, Aidan T.
AU - Loeff, Ina C. D. Schim van der
AU - Barr, Andrew S.
AU - Sanchez-Gonzalez, Amada
AU - Bergamaschi, Laura
AU - Mescia, Federica
AU - Barnes, Josephine L.
AU - Kilich, Eliz
AU - Wilton, Angus de
AU - Saigal, Anita
AU - Saleh, Aarash
AU - Janes, Sam M.
AU - Smith, Claire M.
AU - Gopee, Nusayhah
AU - Wilson, Caroline
AU - Coupland, Paul
AU - Coxhead, Jonathan M.
AU - Kiselev, Vladimir Yu
AU - Dongen, Stijn van
AU - Bacardit, Jaume
AU - King, Hamish W.
AU - Rostron, Anthony J.
AU - Simpson, A. John
AU - Hambleton, Sophie
AU - Laurenti, Elisa
AU - Lyons, Paul A.
AU - Meyer, Kerstin B.
AU - Nikolić, Marko Z.
AU - Duncan, Christopher J.A.
AU - Smith, Kenneth G. C.
AU - Teichmann, Sarah A.
AU - Clatworthy, Menna R.
AU - Marioni, John C.
AU - Göttgens, Berthold
AU - Haniffa, Muzlifah
PY - 2021
DA - April
TI - Single-cell multi-omics analysis of the immune response in COVID-19
JO - Nature Medicine
EP - 916
VL - 27
DO - https://doi.org/10.1038/s41591-021-01329-2
AB - Analysis of human blood immune cells provides insights into the coordinated response to viral infections such as severe acute respiratory syndrome coronavirus 2, which causes coronavirus disease 2019 (COVID-19). We performed single-cell transcriptome, surface proteome and T and B lymphocyte antigen receptor analyses of over 780,000 peripheral blood mononuclear cells from a cross-sectional cohort of 130 patients with varying severities of COVID-19. We identified expansion of nonclassical monocytes expressing complement transcripts (CD16+C1QA/B/C+) that sequester platelets and were predicted to replenish the alveolar macrophage pool in COVID-19. Early, uncommitted CD34+hematopoietic stem/progenitor cells were primed toward megakaryopoiesis, accompanied by expanded megakaryocyte-committed progenitors and increased platelet activation. Clonally expanded CD8+T cells and an increased ratio of CD8+effector T cells to effector memory T cells characterized severe disease, while circulating follicular helper T cells accompanied mild disease. We observed a relative loss of IgA2 in symptomatic disease despite an overall expansion of plasmablasts and plasma cells. Our study highlights the coordinated immune response that contributes to COVID-19 pathogenesis and reveals discrete cellular components that can be targeted for therapy.
PB - Springer Nature
UR - https://hdl.handle.net/2164/20329
KW - Coronavirus (COVID-19)
ER