TY - JOUR
AU - Jansen, Jitske
AU - Reimer, Katharina C.
AU - Nagai, James S.
AU - Varghese, Finny S.
AU - Overheul, Gijs J.
AU - de Beer, Marit
AU - Roverts, Rona
AU - Daviran, Deniz
AU - Fermin, Liline A.S.
AU - Willemsen, Brigith
AU - Beukenboom, Marcel
AU - Djudjaj, Sonja
AU - von Stillfried, Saskia
AU - van Eijk, Larissa E.
AU - Mastik, Mirjam
AU - Bulthuis, Marian
AU - den Dunnen, Wilfred
AU - van Goor, Harry
AU - Hillebrands, Jan Luuk
AU - Triana, Sergio H.
AU - Alexandrov, Theodore
AU - Timm, Marie-Cherelle
AU - van den Berge, Bartholemeus T.
AU - van den Broek, Martijn
AU - Nlandu, Quincy
AU - Heijnert, Joelle
AU - Bindels, Eric M.J.
AU - Hoogenboezem, Remco N.
AU - Mooren, Fieke
AU - Kuppe, Christoph
AU - Kramann, Rafael
PY - 2022
DA - February
TI - SARS-CoV-2 infects the human kidney and drives fibrosis in kidney organoids
JO - Cell Stem Cell
EP - 231
VL - 29
IS - 2
DO - https://doi.org/10.1016/j.stem.2021.12.010
AB - Kidney failure is frequently observed during and after COVID-19, but it remains elusive whether this is a direct effect of the virus. Here, we report that SARS-CoV-2 directly infects kidney cells and is associated with increased tubule-interstitial kidney fibrosis in patient autopsy samples. To study direct effects of the virus on the kidney independent of systemic effects of COVID-19, we infected human-induced pluripotent stem cell-derived kidney organoids with SARS-CoV-2. Single-cell RNA sequencing indicated injury and dedifferentiation of infected cells with activation of profibrotic signaling pathways. Importantly, SARS-CoV-2 infection also led to increased collagen 1 protein expression in organoids. A SARS-CoV-2 protease inhibitor was able to ameliorate the infection of kidney cells by SARS-CoV-2. Our results suggest that SARS-CoV-2 can directly infect kidney cells and induce cell injury with subsequent fibrosis. These data could explain both acute kidney injury in COVID-19 patients and the development of chronic kidney disease in long COVID.
PB - Elsevier
UR - https://aura.abdn.ac.uk/handle/2164/20478
KW - Coronavirus (COVID-19)
ER