TY - JOUR
AU - Wang, Yingxue
AU - Sharma, Parul
AU - Jefferson, Matthew
AU - Zhang, Weijiao
AU - Bone, Ben
AU - Kipar, Anja
AU - Bitto, David
AU - Coombes, Janine L.
AU - Pearson, Timothy
AU - Man, Angela
AU - Zhekova, Alex
AU - Bao, Yongping
AU - Tripp, Ralph A.
AU - Carding, Simon R.
AU - Yamauchi, Yohei
AU - Mayer, Ulrike
AU - Powell, Penny P.
AU - Stewart, James P.
AU - Wileman, Thomas
PY - 2021
DA - February
TI - Non-canonical autophagy functions of ATG16L1 in epithelial cells limit lethal infection by influenza A virus.
JO - EMBO journal
VL - 40
IS - 6
DO - https://doi.org/10.15252/embj.2020105543
AB - Influenza A virus (IAV) and SARS-CoV-2 (COVID-19) cause pandemic infections where cytokine storm syndrome and lung inflammation lead to high mortality. Given the high social and economic cost of respiratory viruses, there is an urgent need to understand how the airways defend against virus infection. Here we use mice lacking the WD and linker domains of ATG16L1 to demonstrate that ATG16L1-dependent targeting of LC3 to single-membrane, non-autophagosome compartments – referred to as non-canonical autophagy – protects mice from lethal IAV infection. Mice with systemic loss of non-canonical autophagy are exquisitely sensitive to low-pathogenicity IAV where extensive viral replication throughout the lungs, coupled with cytokine amplification mediated by plasmacytoid dendritic cells, leads to fulminant pneumonia, lung inflammation and high mortality. IAV was controlled within epithelial barriers where non-canonical autophagy reduced IAV fusion with endosomes and activation of interferon signalling. Conditional mouse models and ex vivo analysis showed that protection against IAV infection of lung was independent of phagocytes and other leucocytes. This establishes non-canonical autophagy in airway epithelial cells as a novel innate defence that restricts IAV infection and lethal inflammation at respiratory surfaces.
PB - Embopress
UR - https://rgu-repository.worktribe.com/output/2035125
KW - Coronavirus (COVID-19)
ER