TY - JOUR
AU - Cantoni, Diego
AU - Mayora-Neto, Martin
AU - Nadesalingam, Angalee
AU - Wells, David A.
AU - Carnell, George W.
AU - Ohlendorf, Luis
AU - Ferrari, Matteo
AU - Palmer, Phil
AU - Chan, Andrew C.Y.
AU - Smith, Peter
AU - Bentley, Emma M.
AU - Einhauser, Sebastian
AU - Wagner, Ralf
AU - Page, Mark
AU - Raddi, Gianmarco
AU - Baxendale, Helen
AU - Castillo-Olivares, Javier
AU - Heeney, Jonathan L.
AU - Temperton, Nigel
PY - 2022
DA - March
TI - Neutralisation hierarchy of SARS-CoV-2 variants of concern using standardised, quantitative neutralisation assays reveals a correlation with disease severity; towards deciphering protective antibody thresholds
JO - Frontiers in Immunology
VL - 13
DO - https://doi.org/10.3389/fimmu.2022.773982
AB - The rise of SARS-CoV-2 variants has made the pursuit to define correlates of protection more troublesome, despite the availability of the World Health Organisation (WHO) International Standard for anti-SARS-CoV-2 Immunoglobulin sera, a key reagent used to standardise laboratory findings into an international unitage. Using pseudotyped virus, we examine the capacity of convalescent sera, from a well-defined cohort of healthcare workers (HCW) and Patients infected during the first wave from a national critical care centre in the UK to neutralise B.1.1.298, variants of interest (VOI) B.1.617.1 (Kappa), and four VOCs, B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma) and B.1.617.2 (Delta), including the B.1.617.2 K417N, informally known as Delta Plus. We utilised the WHO International Standard for anti-SARS-CoV-2 Immunoglobulin to report neutralisation antibody levels in International Units per mL. Our data demonstrate a significant reduction in the ability of first wave convalescent sera to neutralise the VOCs. Patients and HCWs with more severe COVID-19 were found to have higher antibody titres and to neutralise the VOCs more effectively than individuals with milder symptoms. Using an estimated threshold for 50% protection, 54 IU/mL, we found most asymptomatic and mild cases did not produce titres above this threshold.
PB - Frontiers Media
UR - https://eprints.gla.ac.uk/308329/
KW - Coronavirus (COVID-19)
ER