TY - JOUR
AU - Wood, Bayden Robert
AU - Kochan, Kamila
AU - Bedolla, Diana E.
AU - Salazar-Quiroz, Natalia
AU - Grimley, Samantha
AU - Perez-Guaita, David
AU - Baker, Matthew J.
AU - Vongsvivut, Jitraporn
AU - Tobin, Mark
AU - Bambery, Keith
AU - Christensen, Dale
AU - Pasricha, Shivani
AU - Eden, Anthony K.
AU - Mclean, Aaron
AU - Roy, Supti
AU - Roberts, Jason
AU - Druce, Julian
AU - Williamson, Deborah A.
AU - McAuley, Julie
AU - Catton, Mike
AU - Purcell, Damian
AU - Godfrey, Dale
AU - Heruad, Philip
PY - 2021
DA - May
TI - Infrared based saliva screening test for COVID-19
JO - Angewandte International Edition Chemie
DO - https://doi.org/10.1002/anie.202104453
AB - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in an unprecedented need for diagnostic testing that is critical in controlling the spread of COVID-19. We propose a portable infrared spectrometer with purpose-built transflection accessory for rapid point-of-care detection of COVID-19 markers in saliva. Initially, purified virion particles were characterized with Raman spectroscopy, synchrotron infrared (IR) and AFM-IR. A data set comprising 171 transflection infrared spectra from 29 patients testing positive for SARS-CoV-2 by RT-qPCR and 28 testing negative, was modeled using Monte Carlo Double Cross Validation with 50 randomized test and model sets. The sensitivity was 93 % (27/29) and specificity of 82 % (23/28) that included positive samples on the limit of detection for RT-qPCR. Here, we demonstrate a proof-of-concept high throughput infrared COVID-19 test that is rapid, inexpensive, portable and utilizes sample self-collection thus minimizing the risk to healthcare workers and ideally suited to mass screening.
PB - Wiley
UR - https://pureportal.strath.ac.uk/en/publications/infrared-based-saliva-screening-test-for-covid-19
KW - Coronavirus (COVID-19)
ER