TY - JOUR
AU - Morehouse, Zachary P.
AU - Samikwa, Lyson
AU - Proctor, Caleb M.
AU - Meleke, Harry
AU - Kamdolozi, Mercy
AU - Ryan, Gabriella L.
AU - Chaima, David
AU - Ho, Antonia
AU - Nash, Rodney J.
AU - Nyirenda, Tonney S.
PY - 2021
DA - August
TI - Validation of a direct-to-PCR COVID-19 detection protocol utilizing mechanical homogenization: a model for reducing resources needed for accurate testing
JO - PLoS ONE
VL - 16
IS - 8
DO - http://dx.doi.org/10.1371/journal.pone.0256316
AB - Efficient and effective viral detection methodologies are a critical piece in the global response to COVID-19, with PCR-based nasopharyngeal and oropharyngeal swab testing serving as the current gold standard. With over 100 million confirmed cases globally, the supply chains supporting these PCR testing efforts are under a tremendous amount of stress, driving the need for innovative and accurate diagnostic solutions. Herein, the utility of a direct-to-PCR method of SARS-CoV-2 detection grounded in mechanical homogenization is examined for reducing resources needed for testing while maintaining a comparable sensitivity to the current gold standard workflow of nasopharyngeal and oropharyngeal swab testing. In a head-to-head comparison of 30 patient samples, this initial clinical validation study of the proposed homogenization-based workflow demonstrated significant agreeability with the current extraction-based method utilized while cutting the total resources needed in half.
PB - Public Library of Science
UR - http://eprints.gla.ac.uk/249283/
KW - Coronavirus (COVID-19)
ER