TY - JOUR
AU - Lasserre, Perrine
AU - Balansethupathy, Banushan
AU - Vezza, Vincent J.
AU - Butterworth, Adrian
AU - MacDonald, Alexander
AU - Blair, Ewen O.
AU - McAteer, Liam
AU - Hannah, Stuart
AU - Ward, Andrew C.
AU - Hoskisson, Paul A.
AU - Longmuir, Alistair
AU - Setford, Steven
AU - Farmer, Eoghan C. W.
AU - Murphy, Michael E.
AU - Flynn, Harriet
AU - Corrigan, Damion K.
PY - 2022
DA - February
TI - SARS-CoV-2 aptasensor based on electrochemical impedance spectroscopy and low-cost gold electrode substrates
JO - Analytical Chemistry
EP - 2133
VL - 94
IS - 4
DO - https://doi.org/10.1021/acs.analchem.1c04456
AB - SARS-CoV-2 diagnostic practices broadly involve either quantitative polymerase chain reaction (qPCR)-based nucleic amplification of viral sequences or antigen-based tests such as lateral flow assays (LFAs). Reverse transcriptase-qPCR can detect viral RNA and is the gold standard for sensitivity. However, the technique is time-consuming and requires expensive laboratory infrastructure and trained staff. LFAs are lower in cost and near real time, and because they are antigen-based, they have the potential to provide a more accurate indication of a disease state. However, LFAs are reported to have low real-world sensitivity and in most cases are only qualitative. Here, an antigen-based electrochemical aptamer sensor is presented, which has the potential to address some of these shortfalls. An aptamer, raised to the SARS-CoV-2 spike protein, was immobilized on a low-cost gold-coated polyester substrate adapted from the blood glucose testing industry. Clinically relevant detection levels for SARS-CoV-2 are achieved in a simple, label-free measurement format using sample incubation times as short as 15 min on nasopharyngeal swab samples. This assay can readily be optimized for mass manufacture and is compatible with a low-cost meter.
PB - American Chemical Society
UR - https://strathprints.strath.ac.uk/79493/
KW - Coronavirus (COVID-19)
ER