TY - JOUR
AU - Stewart, Alexander
AU - Sinclair, Emma
AU - Ng, Joseph Chi-Fung
AU - O'Hare, Joselli Silva
AU - Page, Audrey
AU - Serangeli, Ilaria
AU - Margreitter, Christian
AU - Orsenigo, Federica
AU - Longman, Katherine
AU - Frampas, Cecile
AU - Costa, Catia
AU - Lewis, Holly-May
AU - Kasar, Nora
AU - Wu, Bryan
AU - Kipling, David
AU - Openshaw, Peter J.M.
AU - Chiu, Christopher
AU - Baillie, J. Kenneth
AU - Scott, Janet T.
AU - Semple, Malcolm G.
AU - Bailey, Melanie J.
AU - Fraternali, Franca
AU - Dunn-Walters, Deborah K.
PY - 2022
DA - May
TI - Pandemic, epidemic, endemic: B cell repertoire analysis reveals unique anti-viral responses to SARS-CoV-2, Ebola and Respiratory Syncytial Virus
JO - Frontiers in Immunology
VL - 13
DO - https://doi.org/10.3389/fimmu.2022.807104
AB - Immunoglobulin gene heterogeneity reflects the diversity and focus of the humoral immune response towards different infections, enabling inference of B cell development processes. Detailed compositional and lineage analysis of long read IGH repertoire sequencing, combining examples of pandemic, epidemic and endemic viral infections with control and vaccination samples, demonstrates general responses including increased use of IGHV4-39 in both Zaire Ebolavirus (EBOV) and COVID-19 patient cohorts. We also show unique characteristics absent in Respiratory Syncytial Virus or yellow fever vaccine samples: EBOV survivors show unprecedented high levels of class switching events while COVID-19 repertoires from acute disease appear underdeveloped. Despite the high levels of clonal expansion in COVID-19 IgG1 repertoires there is a striking lack of evidence of germinal centre mutation and selection. Given the differences in COVID-19 morbidity and mortality with age, it is also pertinent that we find significant differences in repertoire characteristics between young and old patients. Our data supports the hypothesis that a primary viral challenge can result in a strong but immature humoral response where failures in selection of the repertoire risk off-target effects.
PB - Frontiers Media
UR - http://eprints.gla.ac.uk/271168/
KW - Coronavirus (COVID-19)
ER