The impact of hypoxia on B cells in COVID-19 - Repository

Full text

: https://doi.org/10.1016/j.ebiom.2022.103878

Abstract

Background

Prominent early features of COVID-19 include severe, often clinically silent, hypoxia and a pronounced reduction in B cells, the latter important in defence against SARS-CoV-2. This presentation resembles the phenotype of mice with VHL-deficient B cells, in which Hypoxia-Inducible Factors are constitutively active, suggesting hypoxia might drive B cell abnormalities in COVID-19.

Methods

Detailed B cell phenotyping was undertaken by flow-cytometry on longitudinal samples from patients with COVID-19 across a range of severities (NIHR Cambridge BioResource). The impact of hypoxia on the transcriptome was assessed by single-cell and whole blood RNA sequencing analysis. The direct effect of hypoxia on B cells was determined through immunisation studies in genetically modified and hypoxia-exposed mice.

Findings

We demonstrate the breadth of early and persistent defects in B cell subsets in moderate/severe COVID-19, including reduced marginal zone-like, memory and transitional B cells, changes also observed in B cell VHL-deficient mice. These findings were associated with hypoxia-related transcriptional changes in COVID-19 patient B cells, and similar B cell abnormalities were seen in mice kept in hypoxic conditions.

Interpretation

Hypoxia may contribute to the pronounced and persistent B cell pathology observed in acute COVID-19 pneumonia. Assessment of the impact of early oxygen therapy on these immune defects should be considered, as their correction could contribute to improved outcomes.

Rights

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)

Cite as

Kotagiri, P., Mescia, F., Hanson, A., Turner, L., Bergamaschi, L., Peñalver, A., Richoz, N., Moore, S., Ortmann, B., Dunmore, B., Morgan, M., Tuong, Z., Göttgens, B., Toshner, M., Hess, C., Maxwell, P., Clatworthy, M., Nathan, J., Bradley, J., Lyons, P., Burrows, N., Smith, K. & Cambridge Institute of Therapeutic Immunology and Infectious Disease-National Institute of Health Research (CITIID-NIHR) Covid BioResource Collaboration 2022, 'The impact of hypoxia on B cells in COVID-19', eBioMedicine, 77, article no: 103878. https://doi.org/10.1016/j.ebiom.2022.103878

Downloadable citations

HTML BIB RIS

Identifiers

DOI: https://doi.org/10.1016/j.ebiom.2022.103878

Repository URI: https://hdl.handle.net/2164/20597