Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in an unprecedented need for diagnostic testing that is critical in controlling the spread of COVID-19. We propose a portable infrared spectrometer with purpose-built transflection accessory for rapid point-of-care detection of COVID-19 markers in saliva. Initially, purified virion particles were characterized with Raman spectroscopy, synchrotron infrared (IR) and AFM-IR. A data set comprising 171 transflection infrared spectra from 29 patients testing positive for SARS-CoV-2 by RT-qPCR and 28 testing negative, was modeled using Monte Carlo Double Cross Validation with 50 randomized test and model sets. The sensitivity was 93 % (27/29) and specificity of 82 % (23/28) that included positive samples on the limit of detection for RT-qPCR. Here, we demonstrate a proof-of-concept high throughput infrared COVID-19 test that is rapid, inexpensive, portable and utilizes sample self-collection thus minimizing the risk to healthcare workers and ideally suited to mass screening.
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Wood, B., Kochan, K., Bedolla, D., Salazar-Quiroz, N., Grimley, S., Perez-Guaita, D., Baker, M., Vongsvivut, J., Tobin, M., Bambery, K., Christensen, D., Pasricha, S., Eden, A., Mclean, A., Roy, S., Roberts, J., Druce, J., Williamson, D., McAuley, J., Catton, M., Purcell, D., Godfrey, D. & Heruad, P. 2021, 'Infrared based saliva screening test for COVID-19', Angewandte International Edition Chemie. https://doi.org/10.1002/anie.202104453