- Published
- 11 September 2024
- Journal article
Changes in Phenotypic and Molecular Features of Naïve and Central Memory T Helper Cell Subsets following SARS-CoV-2 Vaccination
- Authors
- Source
- Mosavie, M, Rynne, J, Fish, M, Smith, P, Jennings, A, Singh, S, Millar, J, Harvala, H, Mora, A, Kaloyirou, F, Griffiths, A, Hopkins, V, Washington, C, Estcourt, L J, Roberts, D & Shankar-Hari, M 2024, 'Changes in Phenotypic and Molecular Features of Naïve and Central Memory T Helper Cell Subsets following SARS-CoV-2 Vaccination', Vaccines, vol. 12, no. 9, 1040. https://doi.org/10.3390/vaccines12091040
Full text
Abstract
Molecular changes in lymphocytes following SARS-CoV-2 vaccination are incompletely understood. We hypothesized that studying the molecular (transcriptomic, epigenetic, and T cell receptor (TCR) repertoire) changes in CD4 + T cells following SARS-CoV-2 vaccination could inform protective mechanisms and refinement of future vaccines. We tested this hypothesis by reporting alterations in CD4 + T cell subsets and molecular features of CD4 + naïve and CD4 + central memory (CM) subsets between the unvaccinated and vaccinated groups. Compared with the unvaccinated, the vaccinated had higher HLA-DR expression in CD4 + T subsets, a greater number of differentially expressed genes (DEGs) that overlapped with key differentially accessible regions (DARs) along the chromatin linked to inflammasome activation, translation, regulation (of apoptosis, inflammation), and significant changes in clonal architecture beyond SARS-CoV-2 specificity. Several of these differences were more pronounced in the CD4 +CM subset. Taken together, our observations imply that the COVID-19 vaccine exerts its protective effects via modulation of acute inflammation to SARS-CoV-2 challenge.
Rights
© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Cite as
Mosavie, M., Rynne, J., Fish, M., Smith, P., Jennings, A., Singh, S., Millar, J., Harvala, H., Mora, A., Kaloyirou, F., Griffiths, A., Hopkins, V., Washington, C., Estcourt, L., Roberts, D. & Shankar-Hari, M. 2024, 'Changes in Phenotypic and Molecular Features of Naïve and Central Memory T Helper Cell Subsets following SARS-CoV-2 Vaccination', Vaccines, 12(9), article no: 1040. https://doi.org/10.3390/vaccines12091040